ChemRAP uncovers specific mRNA translation regulation via RNA 5'-Pme

ABSTRACT: 5'-end modifications play key roles in determining RNA fates. Phospho-methylation is a non-canonical cap occurring on either 5'-PPP or 5'-P ends. We used ChemRAP, in which affinity purification of cellular proteins with chemically synthesized modified RNAs is coupled to quantitative proteomics, to identify 5'-Pme "readers". We show that 5'-Pme is directly recognized by EPRS, the central subunit of the multisynthetase complex (MSC), through its linker domain, which has previously been involved in key non-canonical EPRS and MSC functions. We further determine that the 5'-Pme writer BCDIN3D regulates the binding of EPRS to specific mRNAs, either at coding regions rich in MSC codons, or around start codons. In the case of LRPPRC (Leucine Rich Pentatricopeptide Repeat Containing), a nuclear-enc

SUBMITTER: Dr. Hélène Ipas 

PROVIDER: S-SCDT-10_1038-S44319-024-00059-Z | biostudies-other |

REPOSITORIES: biostudies-other