RNA polymerase II promotes the organization of chromatin following DNA replication.

ABSTRACT: Understanding how chromatin organisation is duplicated on the two daughter strands is a central question in epigenetics. In mammals, following the passage of the replisome, nucleosomes lose their defined positioning and transcription contributes to their re-organisation. However, whether transcription plays a greater role in the organization of chromatin following DNA replication remains unclear. Here we analysed protein re-association with newly replicated DNA upon inhibition of transcription using iPOND coupled to quantitative mass spectrometry. We show that nucleosome assembly and the re-establishment of most histone modifications are uncoupled from transcription. However, RNAPII acts to promote the re-association of hundreds of proteins with newly replicated chromatin via pathways that

SUBMITTER: Susanne Bandau 

PROVIDER: S-SCDT-10_1038-S44319-024-00085-X | biostudies-other |

REPOSITORIES: biostudies-other