Deep imaging reveals dynamics and signaling in one-to-one pollen tube guidance

ABSTRACT: In the pistil of flowering plants, each ovule usually associates with a single pollen tube for fertilization. This one-to-one pollen tube guidance, which contributes to polyspermy blocking and efficient seed production, is largely different from animal chemotaxis of many sperms to one egg. However, the functional mechanisms underlying the directional cues and polytubey blocks in the depths of the pistil remain unknown. Here, we develop a two-photon live imaging method to directly observe pollen tube guidance in the pistil of Arabidopsis thaliana, clarifying signaling and cellular behaviors in the one-to-one guidance. Ovules are suggested to emit multiple signals for pollen tubes, including an integument-dependent directional signal that reaches the inner surface of the septum and adhesion

SUBMITTER: Dr. Yoko Mizuta 

PROVIDER: S-SCDT-10_1038-S44319-024-00151-4 | biostudies-other |

REPOSITORIES: biostudies-other