Conserved role of hnRNPL in alternative splicing of epigenetic modifiers enables B cell activation

ABSTRACT: The multifunctional RNA-binding protein hnRNPL is implicated in antibody class switching but its broader function in B cells is unknown. Here, we show that hnRNPL is essential for B cell activation, and germinal center and antibody responses. Upon activation, hnRNPL-deficient B cells show proliferation defects and increased apoptosis. Comparative analysis of RNA-seq data from activated B cells and further eight hnRNPL-depleted cell types reveals common effects on MYC and E2F transcriptional programs required for proliferation. Notably, while individual gene expression changes are cell type specific, several alternative splicing events affecting histone modifiers like KDM6A and SIRT1, are conserved across cell types. Moreover, hnRNPL-deficient B cells show global changes in H3K27me3 and H3K

SUBMITTER: Mr. Poorani, Ganesh Subramani 

PROVIDER: S-SCDT-10_1038-S44319-024-00152-3 | biostudies-other |

REPOSITORIES: biostudies-other