Senescent endothelial cells promote pathogenic neutrophil trafficking in inflamed tissues

ABSTRACT: Cellular senescence is a hallmark of advanced age and a major instigator of numerous inflammatory pathologies. Whilst endothelial cell (EC) senescence is aligned with defective vascular functionality, its impact on fundamental inflammatory responses in vivo at single cell level remain unclear. To directly investigate the role of EC senescence on dynamics of neutrophil-venular wall interactions, we applied high resolution confocal intravital microscopy to inflamed tissues of an EC-specific progeroid mouse model, characterized by profound indicators of EC senescence. Progerin-expressing ECs supported prolonged neutrophil adhesion and crawling in a cell autonomous manner that additionally mediated neutrophil-dependent microvascular leakage. Transcriptomic and immunofluorescence analysis of in

SUBMITTER: Dr. Loïc Rolas 

PROVIDER: S-SCDT-10_1038-S44319-024-00182-X | biostudies-other |

REPOSITORIES: biostudies-other