Local translatome sustains synaptic function in impaired Wallerian degeneration

ABSTRACT: After injury, severed axons separated from their somas activate programmed axon degeneration, a conserved pathway to initiate their degeneration within a day. Conversely, severed projections deficient in programmed axon degeneration remain morphologically preserved with functional synapses for weeks to months after axotomy. How this synaptic function is sustained remains currently unknown. Here, we show that dNmnat over-expression attenuates programmed axon degeneration in distinct neuronal populations. Severed projections remain morphologically preserved for weeks. When evoked, they elicit a postsynaptic behavior, a readout for preserved synaptic function. We used ribosomal pulldown to isolate the translatome from these projections one week after axotomy. Translatome candidates of enriche

SUBMITTER: Dr. Maria Paglione 

PROVIDER: S-SCDT-10_1038-S44319-024-00301-8 | biostudies-other |

REPOSITORIES: biostudies-other