The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-kappaB / RELA

ABSTRACT: The protein interactome of p65 / RELA, the most active subunit of the transcription factor (TF) NF-kappaB, has not been previously determined in living cells. Using p65-miniTurbo fusion proteins and biotin tagging, we identify > 350 RELA interactors from untreated and IL-1α-stimulated cells, including many TFs (47 % of all interactors) and > 50 epigenetic regulators belonging to different classes of chromatin remodeling complexes. A comparison with the interactomes of two point mutants of p65 reveals that the interactions primarily require intact dimerization rather than DNA binding properties. A targeted RNAi screen for 38 interactors and subsequent functional transcriptome and bioinformatics studies identify gene regulatory (sub)networks, each controlled by RELA in combination with

SUBMITTER: Lisa Leib 

PROVIDER: S-SCDT-10_1038-S44319-024-00339-8 | biostudies-other |

REPOSITORIES: biostudies-other