Cytosolic CRISPR RNAs for efficient application of RNA-targeting CRISPR-Cas systems

ABSTRACT: Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technologies have evolved rapidly over the past decade with the continuous discovery of new Cas systems. In particular, RNA-targeting CRISPR-Cas13 proteins are promising single-effector systems to regulate target mRNAs without altering genomic DNA, yet the current Cas13 systems are restrained by suboptimal efficiencies. Here, we show that U1 promoter-driven CRISPR RNAs (crRNAs) increase the efficiency of various applications, including RNA knockdown and editing, without modifying the Cas13 protein effector. We confirm that U1-driven crRNAs are exported into the cytoplasm, while conventional U6 promoter-driven crRNAs are mostly confined to the nucleus. Furthermore, we reveal that the end positio

SUBMITTER: Ezra C.K. Cheng 

PROVIDER: S-SCDT-10_1038-S44319-025-00399-4 | biostudies-other |

REPOSITORIES: biostudies-other