MTOR controls ependymal differentiation by targeting alternative cell cycle and centrosomal proteins

ABSTRACT: Ependymal cells are multiciliated glial cells lining the ventricles of the mammalian brain. Their differentiation from progenitors involves cell enlargement and progresses through centriole amplification phases and ciliogenesis. These phases are accompanied by the sharp up-regulation of mTORC1, a master regulator of macromolecule biosynthesis and cell growth, whose function in ependymal cell differentiation is unknown. We demonstrate that mTORC1 inhibition by rapamycin preserves the progenitor pool by reinforcing quiescence and preventing alternative cell cycle progression for centriole amplification. Overexpressing E2F4 and MCIDAS circumvents mTORC1-regulated processes, enabling centriole amplification despite rapamycin, and enhancing mTORC1 activity through positive feedback. Acute rapam

SUBMITTER: Mario Pende 

PROVIDER: S-SCDT-10_1038-S44319-025-00460-2 | biostudies-other |

REPOSITORIES: biostudies-other