Targeted Protein Degradation in Escherichia coli using CLIPPERs

ABSTRACT: New, universal tools for targeted protein degradation in bacteria can help to accelerate protein function studies and antimicrobial research. We describe a new method for degrading bacterial proteins using plasmid-encoded degrader peptides which deliver target proteins for degradation by a highly conserved ClpXP protease. We demonstrate the mode of action of the degraders on a challenging essential target, GroEL. The studies in bacteria are complemented by in vitro binding and structural studies. Expression of degrader peptides results in a temperature-dependent growth inhibition and depletion of GroEL levels over time. The reduction of GroEL levels is accompanied by dramatic proteome alterations. The presented method offers a new alternative approach for regulating protein levels in bacte

SUBMITTER: Matylda, Anna Izert-Nowakowska 

PROVIDER: S-SCDT-10_1038-S44319-025-00510-9 | biostudies-other |

REPOSITORIES: biostudies-other