Inhibition of the minor spliceosome restricts the growth of a broad spectrum of cancers

ABSTRACT: Minor splicing is an under-appreciated splicing system required for the correct expression of approximately 700 genes in the human genome. This small subset of genes (0.35%) harbour introns containing non-canonical splicing sequences that are recognised uniquely by the minor spliceosome and cannot be processed by the major spliceosome. Using in vivo zebrafish and mouse cancer models, we show that heterozygous expression of Rnpc3, encoding a unique protein component of the minor spliceosome, restricts the growth and survival of liver, lung and gastric tumours without impacting healthy cells. RNPC3 knockdown in human lung cancer-derived A549 cells also impairs cell proliferation and RNA-seq analysis reveals a robust and selective disruption to minor intron splicing and transcription-wide eff

SUBMITTER: Karen Doggett 

PROVIDER: S-SCDT-10_1038-S44319-025-00511-8 | biostudies-other |

REPOSITORIES: biostudies-other