Structural obstruction to full DNA replication in terminally differentiated skeletal muscle cells

ABSTRACT: Terminal cell differentiation is often associated with permanent withdrawal from proliferation, termed the postmitotic state. Though widespread among vertebrates and determinant for their biology, the molecular underpinnings of this state are poorly understood. Postmitotic skeletal muscle myotubes can be induced to reenter the cell cycle; however, they generally die as a result of their inability to complete DNA replication. Here, we explore the causes of such incompetence. Genomic hybridization of newly synthesized DNA shows that the replicative failure does not concern specific genomic regions, but can stochastically affect any of them. Myoblast and myotube nuclei are incubated in replicative Xenopus egg extract, which provides a full DNA replication machinery. While myoblast nuclei atta

SUBMITTER: Marco Crescenzi 

PROVIDER: S-SCDT-10_1038-S44319-025-00554-X | biostudies-other |

REPOSITORIES: biostudies-other