The invasion pore induced by Toxoplasma gondii

ABSTRACT: The parasite Toxoplasma gondii invades its host cell only after secreting proteins such as invasion-requisite RON2 that inserts into the host cell membrane to establish the moving junction. Electrophysiological recordings at sub-200 µs resolution showed a transient increase in host cell membrane conductance following parasite exposure. Transients always preceded invasion, but parasites depleted of RON2 generated transients without invading, thus RON2 is not essential for transient generation. Time-series analysis developed here and applied to the 910,000 data point transient dataset revealed multiple quantal conductance changes in the parasite-induced transient, consistent with rapid insertion, then slower removal, blocking, or inactivation of potential pore components. Quantal steps for w

SUBMITTER: Yuto Kegawa 

PROVIDER: S-SCDT-10_1038-S44319-025-00565-8 | biostudies-other |

REPOSITORIES: biostudies-other