An Argonaute protein traffics from nematode to mouse and is a vaccine against parasitic nematodes

ABSTRACT: Argonautes are ancient proteins with well-characterised functions in cell-autonomous gene regulation and genome defense but less clear roles in non-cell-autonomous processes. Extracellular Argonautes have been reported across plants, animals and protozoa yet their biochemical and functional properties remain elusive. Here we demonstrate that an extracellular Argonaute (exWAGO) released by the rodent-infective nematode Heligmosomoides bakeri is detectable inside mouse cells during the natural infection. We show that exWAGO is released from H.bakeri in both vesicular and non-vesicular forms that have different resistances to proteolysis, different accessibilities to antibodies and associate with different subsets of secondary siRNAs. Using recombinant exWAGO protein we demonstrate that non-v

SUBMITTER: Kyriaki Neophytou 

PROVIDER: S-SCDT-10_1038-S44319-025-00620-4 | biostudies-other |

REPOSITORIES: biostudies-other