Fluorescent protein and peptide tags alter condensate formation and dynamics in vivo and in vitro

ABSTRACT: Fluorescent proteins and peptide tags are essential tools in cellular biology, but can alter the biochemical and physiological behavior of target proteins. Biomolecular condensates, which have emerged as key principles of cellular organization, are suggested to provide robustness to cells, yet they can also respond sensitively to small changes in environmental conditions including tags. Here, we investigate the effects of over twenty widely used tags on condensate formation in vitro, in cells, in various model organisms and by computational modelling. We find that tagging strongly influences condensation for some proteins, while others remain unaffected. Effects vary, with some tags enhancing and others decreasing condensation, with the outcome depending on the protein being tagged. Coarse

SUBMITTER: Kerstin Dörner 

PROVIDER: S-SCDT-10_1038-S44319-025-00626-Y | biostudies-other |

REPOSITORIES: biostudies-other