Functional profiling and visualization of the sphingolipid metabolic network in vivo

ABSTRACT: Sphingolipids govern diverse cellular processes; their dysregulation underlies numerous diseases. Despite extensive characterizations, understanding the orchestration of the sphingolipid network within living organisms remains challenging. We established a versatile genetic platform of CRISPR-engineered reporters of 52 sphingolipid regulators, recapitulating endogenous gene activity and protein distribution. This platform further allows conditional protein degradation for functional characterizations. Additionally, we developed the biosensor OlyAw to detect ceramide phosphoethanolamine and visualize membrane raft dynamics in vivo. Using this platform, we established comprehensive profiles of the sphingolipid metabolic network in the brain at the transcription and translation levels. The hi

SUBMITTER: Fei-Yang Tzou 

PROVIDER: S-SCDT-10_1038-S44319-025-00632-0 | biostudies-other |

REPOSITORIES: biostudies-other