Gim3 buffers and potentiates de novo mutations that affect fluconazole susceptibility in yeast

ABSTRACT: Gim3 is an evolutionarily conserved component of the prefoldin chaperone complex, involved in protein folding. We previously found that GIM3 genetically interacts with many de novo mutations in Saccharomyces cerevisiae. Removing GIM3 from mutagenized S. cerevisiae cells significantly affected the fitness effect of mutations. This indicates that Gim3 might change the evolutionary impact of de novo mutations by either buffering (hiding) or potentiating (increasing) their phenotypic effects, depending on the environmental or genetic context. Here, we investigated Gim3's role in shaping the evolutionary fate of de novo mutations under fluconazole stress, an antifungal drug used to combat fungal infections. Applying both strong and moderate fluconazole stress in the presence or absence of GIM3

SUBMITTER: Mohammed, T. Tawfeeq 

PROVIDER: S-SCDT-10_1038-S44319-026-00702-X | biostudies-other |

REPOSITORIES: biostudies-other