Phosphorylation of Xenopus M18BP1 governs centromeric localization and CENP-A nucleosome assembly

ABSTRACT: Eukaryotic chromosome segregation requires attachment of chromosomes to microtubules through the kinetochore so that chromosomes can align and move in mitosis. Kinetochores assemble on the centromere which is epigenetically defined by the histone H3 variant CENtromere Protein A (CENP-A). During DNA replication CENP-A is equally divided between replicated chromatids and new CENP-A nucleosomes are re-assembled during the subsequent G1 phase. How cells regulate the cell cycle timing of CENP-A assembly is a central question in the epigenetic maintenance of centromeres. CENP-A nucleosome assembly requires the Mis18 complex (Mis18α, Mis18β, and M18BP1), whose localization to centromeres occurs between metaphase and G1. Here, we define a new regulatory mechanism that works through phosphorylation

SUBMITTER: Rae, R Brown 

PROVIDER: S-SCDT-10_1038-S44319-026-00714-7 | biostudies-other |

REPOSITORIES: biostudies-other