Transient telomere uncapping triggers telomeric and subtelomeric rearrangements

ABSTRACT: Telomeres cap the extremities of linear chromosomes and prevent their detection as DNA damage. Telomere uncapping poses a profound threat to genome integrity, yet the immediate consequences of transient uncapping remain unclear. In Saccharomyces cerevisiae, the Cdc13-Stn1-Ten1 complex limits resection, preventing DNA damage checkpoint activation. Here, using the temperature-sensitive cdc13-1 allele, we demonstrate that transient telomere uncapping rapidly induces extensive genomic rearrangements despite a functional DNA damage checkpoint. Two distinct rearrangement signatures are observed in surviving cells: recombination of the subtelomeric region mostly involving the Y' elements, and massively elongated telomeres up to 10 kb, a ~30-fold increase. Long-read sequencing evidences Y' element

SUBMITTER: Liébaut Dudragne 

PROVIDER: S-SCDT-10_1038-S44319-026-00717-4 | biostudies-other |

REPOSITORIES: biostudies-other