Relevance of the antibody Fc fragment and epitope valency in protection against malaria sporozoites

ABSTRACT: Protection against infection by Plasmodium falciparum sporozoites is mediated in part by antibodies that recognize the repeated (NPNA)3 epitopes of the circumsporozoite protein (CSP). To evaluate the role that the antibody Fc region plays in neutralizing sporozoite infectivity, we assess the protective efficacy of Fab fragments obtained from protective human monoclonal antibodies and use antibodies bearing the LALA and KA mutations known to reduce Fc functions. To determine the impact that multiple-antibody binding to CSP on protection, we use sporozoites from genetically modified P. berghei strains expressing a CSP containing different numbers of (NPNA)3 epitopes. Our results indicate that Fab fragments and Fc-mutated antibodies have a protective efficacy comparable to intact antibodies,

SUBMITTER: Fidel Zavala 

PROVIDER: S-SCDT-10_1038-S44319-026-00788-3 | biostudies-other |

REPOSITORIES: biostudies-other