Developing a Mobile RNA Delivery System for Grafting Trait Improvement

ABSTRACT: Systemic RNA movement offers a route for non-transgenic trait improvement by grafting, but progress has been limited by the lack of reliable tools to identify mobile RNA elements. Here, we report a fluorescence-aptamer-based live-imaging platform for screening mobile RNAs and mapping mobility elements. In a transient screening assay, live imaging of 100 RNA-seq-predicted candidates detects three reproducibly mobile mRNAs, CAT3, CK1 and GAI, under our assay conditions, with GAI mRNA exhibiting the highest bidirectional mobility. Truncation analysis defines two independent 30-nt cis-elements, GME1 in the coding region and GME2 in the 3′UTR, with GME2 mediating higher transport rate and mobility rate. A tandem 2×GME2 cassette in the 3′UTR functions as a high-capacity RNA delivery module and,

SUBMITTER: Yiran Tao 

PROVIDER: S-SCDT-10_1038-S44319-026-00819-Z | biostudies-other |

REPOSITORIES: biostudies-other