Validation of the mRNA epitranscriptome: SCARPET reveals that mapped m1A sites are inosine

ABSTRACT: A major goal in epitranscriptomics is to define the diversity of modified nucleotides in mRNA. Most candidate sites are discovered using transcriptome-wide mapping methods that rely on antibody enrichment or modification-induced misincorporations during cDNA synthesis. However, these maps are rarely verified biochemically, and in some cases have yielded conflicting conclusions, most notably for N1-methyladenosine (m1A). Initial transcriptome-wide maps reported thousands of internal m1A sites while later studies disputed these findings and showed that these sites lacked the expected m1A-induced misincorporations. More recently, an evolved reverse transcriptase that detects m1A again suggested that internal m1A might be widespread. These divergent results underscore the need for biochemical

SUBMITTER: Rohit Nalavade 

PROVIDER: S-SCDT-10_1038-S44319-026-00840-2 | biostudies-other |

REPOSITORIES: biostudies-other