Foxe1 deficiency impairs thyroid fate while supporting a lung differentiation

ABSTRACT: Patterning of mammalian endoderm into lung and thyroid lineages depends upon a correct early expression of a homeobox domain-containing transcription factor, Nkx2-1. However, the gene networks distinguishing the differentiation of those lineages remain largely unknown. In this work, by using mouse stem cell lines, scRNA-seq, and transcriptomic and chromatin accessibility profiling, we show that Foxe1 knockout impairs Nkx2-1+ cell differentiation and maturation into thyroid follicular-like cells. Concomitantly, a subset of Foxe1 null/Nkx2-1+ cells follow a lung epithelial differentiation program and form lung-like organoids harboring cells transcriptionally similar to mouse fetal lung types. Chromatin analyses reveal that, while accessibility at the Pax8 locus is reduced, loci associated wi

SUBMITTER: Barbara, F Fonseca 

PROVIDER: S-SCDT-10_1038-S44319-026-00841-1 | biostudies-other |

REPOSITORIES: biostudies-other