Novel CDK-independent function of CDC25 phosphatases in mRNA translation

ABSTRACT: Molecular and functional networks driving coordination between cell cycle and mRNA translation remain to be explored. Here, we use mass spectrometry-based proteomics to comprehensively investigate the interactome and phosphoproteome of the cell cycle regulator CDC25A. We identify actors of mRNA regulation, such as RNA-binding proteins and translation factors, as interacting partners of CDC25A. CDC25A overexpression increases global translation, whereas catalytic inactivation or pharmacological inhibition decreases protein synthesis. A Cyclin Dependent Kinase (CDK) interaction-deficient mutant of CDC25A also enhances translation, indicating a CDK-independent role. Our results further reveal an interplay between CDC25A and CDC25B whereby down-regulation of CDC25A leads to compensatory overex

SUBMITTER: Sauyeun Shin 

PROVIDER: S-SCDT-10_1038-S44319-026-00852-Y | biostudies-other |

REPOSITORIES: biostudies-other