Cumulative microscopy reveals cellular states in fibroblasts from patients with genetic disorders

ABSTRACT: Analysis of cellular states and signaling trajectories can provide insights into causes of disease. We developed cumulative microscopy, a method to perform cyclical imaging without elution or quenching steps. Cumulative microscopy computationally extracts individual signals from accumulating fluorescence during sequential imaging. We use cumulative microscopy to quantitatively assess cell cycle and stress markers in individual primary fibroblasts from patients with rare genetic proliferative disorders with increased cancer risk. Neural network-based analysis of cumulative microscopy data suggests that cells from patients with Cartilage-hair hypoplasia (CHH), but not Mulibrey Nanism (MUL), show replication stress. We analyze cell states and cell trajectories and find that a subset of cells

SUBMITTER: Karen Akopyan 

PROVIDER: S-SCDT-10_1038-S44319-026-00859-5 | biostudies-other |

REPOSITORIES: biostudies-other