3D chromatin organisation contributes to regulatory logic in differentiating lymphatic endothelium

ABSTRACT: Gene activation and repression is an integral part of embryonic development and tissue formation. Changes in chromatin organisation dictate accessibility to gene regulatory elements, controlling gene expression. Although several molecular regulators of lymphatic endothelial cell (LEC) development have been identified, the role of chromatin organisation in the acquisition of LEC identity remains unclear. In this study, we combined Hi-C and ATAC-sequencing to map 3D chromatin architecture and accessibility in LECs and blood endothelial cells (BECs). We identified cell type-specific topologically associating domains (TADs), and discovered TAD boundaries changes and differentially segregating enhancers in lymphatic-associated loci, such as prox1a and tbx1. Our multi-omic approach also defined

SUBMITTER: Virginia Panara 

PROVIDER: S-SCDT-10_1038-S44319-026-00895-1 | biostudies-other |

REPOSITORIES: biostudies-other