Orphan GPR84 facilitates uropod de-adhesion at multiple steps during leukocyte extravasation

ABSTRACT: Leukocyte migration through venular walls is an essential component of effective immunity. While the key molecular players driving the initial steps of this response are known, the terminating signals remain unclear. Here, we identify a conserved role for GPR84 family GPCRs in successful completion of the final stages of leukocyte extravasation through acutely inflamed vessels. The integration of high resolution intravital imaging with cell-specific genetics reveals that deficiency of GPR84 orthologues in mice and Drosophila, results in flawed detachment of transmigrating immune cells from vessel walls. Mechanistically, transcriptomics reveals GPR84-deficient neutrophils exhibit defective actin cytoskeletal regulation and cellular adhesion/de-adhesion. Consistent with this, our fly-murine

SUBMITTER: Clare Latta 

PROVIDER: S-SCDT-10_1038-S44319-026-00923-0 | biostudies-other |

REPOSITORIES: biostudies-other