LC3 forms functional nanoclusters on autophagosome

ABSTRACT: Autophagosome biogenesis depends on the coordinated action of proteins and lipids. However, how LC3B organizes at high density and contributes to autophagy-associated functions remains unclear. Using molecular dynamics simulations and super-resolution microscopy, we show that LC3B self-assembles into higher-order nanoclusters approximately 150 nm in size. These clusters form spatially distinct LC3B "islands" on the autophagosome membrane and show a preferential association with phosphatidylinositol-3-phosphate (PI) lipids. Molecular analysis of 296 structural clusters reveals a putative LC3B homo-clustering interface characterized by a distinct alternating hydrophobic-polar motif. To experimentally validate this interface, we generated four LC3B mutants targeting the clustering motif. STOR

SUBMITTER: Saman Fatihi 

PROVIDER: S-SCDT-10_1038-S44319-026-00931-0 | biostudies-other |

REPOSITORIES: biostudies-other