Nucleolar reorganization upon stress depends on physicochemical changes due to reduced nascent rRNA synthesis

ABSTRACT: Successive maturation of ribosomal subunits occurs through multilayered phase-separated structures of the cell nucleolus. The spatio-functional relationship between transcription of rRNA and nucleolar substructures, and how this adapts to cellular stress remain incompletely understood. In this study, we resolve the sub nucleolar structures using expansion microscopy to reveal ordered structures of fibrillar center (FC) and dense fibrillar component (DFC) domains as nested shells, which are reorganized upon cellular stress like DNA damage or RNA polymerase I (RNAPI) inhibition. Direct visualization of nascent (5' ETS) and mature (28S or 18S) rRNA suggests that rRNA synthesis is a critical regulator of nucleolar size and organization. Nucleolar reorganization upon stress emerges to be a dire

SUBMITTER: Sinjini Ghosh 

PROVIDER: S-SCDT-10_1038-S44319-026-00932-Z | biostudies-other |

REPOSITORIES: biostudies-other