Mammalian MemPrep establishes the lipid composition of ER membranes in HEK293T cells

ABSTRACT: The endoplasmic reticulum (ER) forms a dynamic network of sheets and tubules, whose lipid composition remains incompletely defined. Using an optimized MemPrep workflow, we establish a high‑confidence lipidome of the mammalian ER and selectively enrich membrane vesicles originating from ER tubules as a major ER subdomain. Quantitative lipidomics show ER membranes are dominated by phosphatidylcholine and mono‑unsaturated glycerophospholipids, consistent with a highly compressible bilayer. Although proteomics suggests a functional specialization of ER tubules and an enrichment of tubule-associated proteins, the lipidome of an ER tubule-enriched isolate is indistinguishable from the general ER, indicating that principal ER architectures share a common lipid composition. Integration of lipidomi

SUBMITTER: Aamna Jain 

PROVIDER: S-SCDT-10_1038-S44319-026-00952-9 | biostudies-other |

REPOSITORIES: biostudies-other