Self-organization of Drosophila chromatin architecture in a cell-free system

ABSTRACT: Metazoan genomes are organized into loops and domains that support long-range regulatory interactions. Although cohesin-mediated loop extrusion and architectural DNA-binding proteins are central to current models of genome organization, how higher-order chromatin structures emerge remains incompletely understood. Early Drosophila melanogaster embryogenesis provides a unique opportunity to study chromatin folding in the near absence of transcription, but dissecting the underlying mechanisms in vivo is challenging. Here, we establish an in vitro system that reconstitutes chromatin using extracts from syncytial embryos. Nucleosome mapping and Micro-C reveal that loops, trans interactions and topologically associating domains (TADs) emerge from soluble extract components. While some structur

SUBMITTER: Muhunden Jayakrishnan 

PROVIDER: S-SCDT-10_1038-S44319-026-00969-0 | biostudies-other |

REPOSITORIES: biostudies-other