Gαi2 promotes microtubule-driven incorporation of neurofilaments to stabilise nascent axons in vivo

ABSTRACT: Axon initiation is a critical step in the establishment of neuronal morphology. However, the mechanisms regulating stable axon initiation during embryonic development remain poorly understood. Here, using fixed-tissue super-resolution and high-resolution live-tissue imaging in the developing chick spinal cord, we reveal that stable axon initiation is characterised by microtubule-directed neurofilament accumulation in the emerging axon and identify the multifunctional G-protein Gαi2 as a regulator of this process. Before axon initiation, microtubule network orientation shifts towards the site of the future axon where coiled Gαi2-associated neurofilaments accumulate asymmetrically before unfurling into the initiating axon behind microtubules. Crucially, Gαi2 is associated with neurofilaments

SUBMITTER: Victoria Higgs 

PROVIDER: S-SCDT-10_1038-S44319-026-00971-6 | biostudies-other |

REPOSITORIES: biostudies-other