Systematic Identification of 20S Proteasome Substrates

ABSTRACT: For years, proteasomal degradation was predominantly attributed to the ubiquitin-26S pathway. However, it is now evident that the core 20S proteasome can independently target proteins for degradation. With approximately half of cellular proteasomes comprising free 20S complexes, this degradation mechanism is not rare. Identifying 20S-specific substrates is challenging due to the dual-targeting of some proteins to either 20S or 26S proteasomes and the non-specificity of proteasome inhibitors. Consequently, knowledge of 20S proteasome substrates relies on limited hypothesis-driven studies. To comprehensively explore 20S proteasome substrates, we employed advanced mass spectrometry, along with biochemical and cellular analyses. This systematic approach revealed hundreds of 20S proteasome subs

SUBMITTER: Dr Monika Pepelnjak 

PROVIDER: S-SCDT-10_1038-S44320-024-00015-Y | biostudies-other |

REPOSITORIES: biostudies-other