Alternative proteoforms and proteoform-dependent assemblies in humans and plants

ABSTRACT: Variability of proteins at the sequence level creates an enormous potential for proteome complexity. Exploring this complexity is an ongoing goal in biology. Here, we survey human and plant bottom-up native proteomics data for protein truncation variants, where substantial regions of the full-length protein are missing from an observed protein product. In humans, Arabidopsis, and Chlamydomonas, approximately one percent of proteins show a short form, which we can assign by comparison to RNA isoforms as likely deriving from transcript-directed processes or limited proteolysis. While some detected protein fragments align with known splice forms and protein cleavage events, multiple examples are previously undescribed, such as fibrocystin proteolysis and nuclear translocation in a green alga.

SUBMITTER: Dr Claire, D McWhite 

PROVIDER: S-SCDT-10_1038-S44320-024-00048-3 | biostudies-other |

REPOSITORIES: biostudies-other