High-throughput protein characterization by complementation using DNA barcoded fragment libraries

ABSTRACT: Our ability to predict, control, or design biological function is fundamentally limited by poorly annotated gene function. This can be particularly challenging in non-model systems. Accordingly, there is motivation for new high-throughput methods for accurate functional annotation. Here, we use complementation of auxotrophs and DNA barcode sequencing (Coaux-Seq) to enable high-throughput characterization of protein function. Fragment libraries from eleven genetically diverse bacteria were tested in twenty different auxotrophic strains of Escherichia coli to identify genes complimenting missing biochemical activity. Although assay effectiveness ranged with respect to source genome (41% recovery), even distant E. coli relatives Bacillus subtilis and Bacteroides thetaiotaomicron showed succes

SUBMITTER: Dr Bradley, W. Biggs 

PROVIDER: S-SCDT-10_1038-S44320-024-00068-Z | biostudies-other |

REPOSITORIES: biostudies-other