A single-cell multiomics roadmap of zebrafish spermatogenesis reveals regulatory principles of male germline formation

ABSTRACT: Spermatogenesis is the process by which spermatozoa are produced in the testes. Beyond facilitating the transmission of genetic information, spermatogenesis also provides a framework for transgenerational inheritance of gene-regulatory states. While extensively studied in mammals, our understanding of spermatogenesis in anamniotes remains limited. Here we present a comprehensive single-cell multiomics resource, combining single-cell RNA sequencing and single-cell chromatin accessibility profiling, with base-resolution DNA methylome analysis of sorted germ cell populations from zebrafish testes. We identify major germ cell types involved in zebrafish spermatogenesis as well as key drivers associated with these transcriptional states. Moreover, we describe localised DNA methylation changes a

SUBMITTER: Ana, M. Burgos-Ruiz 

PROVIDER: S-SCDT-10_1038-S44320-025-00157-7 | biostudies-other |

REPOSITORIES: biostudies-other