Engineering mammalian cells for detection and treatment of cardiac injury

ABSTRACT: Early detection of myocardial abnormalities or other ischemic heart diseases is critical for effective treatment. Here, we engineered a cell-based system to sense cardiac troponin I (cTnI), an early marker of AMI, and respond by releasing a thrombolytic agent. To detect cTnI, we engineered a chimeric troponin receptor (TropR) that contains extracellular single-chain variable fragments (scFvs) and signals via intracellular domains of IL6RB, EGFR, FGFR1, FGFR2b or VEGFR2 that are associated with cardioprotective signaling. cTnI-dependent TropR functionality was confirmed in human cell lines as well as iPSC-derived cardiomyocytes, and enabled rapid, reversible, tunable control of gene expression via synthetic-signaling-specific promoters. Monoclonal cell lines for cTnI-induced secretion of th

SUBMITTER: Yaqing Si 

PROVIDER: S-SCDT-10_1038-S44320-025-00161-X | biostudies-other |

REPOSITORIES: biostudies-other