Cell cycle-dependent protein dynamics in budding yeast resolved by deconvolution of bulk proteomics

ABSTRACT: The cell division cycle is characterised by oscillatory dynamics in regulatory mechanisms and biosynthesis, coordinated with genome replication and segregation. To understand these dynamics, quantitative cell cycle-dependent protein concentration data are essential. Unfortunately, accurately resolving cell cycle-dependent protein dynamics is challenging because single-cell proteomics is currently infeasible and bulk proteomics requires - inherently imperfect - cell synchronisation. Here, we developed a computational method to deconvolve cell cycle-dependent protein concentration dynamics and applied it to new budding yeast bulk proteome data. Key to this method was a yeast population model, parameterised with experimental cell cycle-progression and volume growth data, for quantifying the d

SUBMITTER: Andre, J Zylstra 

PROVIDER: S-SCDT-10_1038-S44320-026-00241-6 | biostudies-other |

REPOSITORIES: biostudies-other