Histidine-rich glycoprotein modulates neutrophils and thrombolysis-associated hemorrhagic transformation

ABSTRACT: Intravenous thrombolysis using recombinant tissue plasminogen activator (tPA) remains the primary treatment for patients with acute ischemic stroke (AIS). However, the mechanism of tPA-related hemorrhagic transformation (HT) remains poorly understood. Elevation of histidine-rich glycoprotein (HRG) expression was detected by nano-liquid chromatography tandem mass spectrometry at 1 h following tPA infusion as compared to baseline prior to tPA infusion (discovery cohort, n=10), which was subsequently confirmed in a validation cohort (n=157) by ELISA. Surprisingly, no elevation of HRG was detected in individuals who subsequently developed HT. During in vitro experiments, HRG reduced neutrophil NETosis, inflammatory cytokine production, and migration across the blood-brain barrier induced by tP

SUBMITTER: Wei Jiang 

PROVIDER: S-SCDT-10_1038-S44321-024-00117-Y | biostudies-other |

REPOSITORIES: biostudies-other