Oncolytic viruses expressing MATEs facilitate target-independent T-cell activation in tumors

ABSTRACT: Oncolytic viruses (OV) expressing bispecific T-cell engagers (BiTEs) are promising tools for tumor immunotherapy but the range of target tumors is limited. To facilitate effective T-cell stimulation with broad-range applicability, we established membrane-associated T-cell engagers (MATEs) harboring the protein transduction domain of the HIV-Tat protein to achieve non-selective binding to target cells. In vitro, MATEs effectively activated murine T-cells and improved killing of MC38 colon carcinoma cells. Similarly, humanized MATEs activated T-cells in PBMCs from human donors. In MC38-tumors in mice, MATE-expression by the oncolytic adenovirus Ad5/11 facilitated intratumoral T-cell activation, reduced tumor growth and prolonged survival accompanied by infiltration of tumor-directed CD8+ T-c

SUBMITTER: Malin Peter 

PROVIDER: S-SCDT-10_1038-S44321-024-00187-Y | biostudies-other |

REPOSITORIES: biostudies-other