DNA Methylation Changes in COVID-19 Link to Long-Term Transcriptional Dysregulation in Airway Cells

ABSTRACT: Molecular changes underlying the persistent health effects after SARS-CoV-2 infection remain poorly understood. To discern the gene regulatory landscape in the upper respiratory tract of COVID-19 patients, we performed enzymatic DNA methylome and single-cell RNA sequencing in nasal cells of COVID-19 patients (n=19, scRNA-seq n=14) and controls (n=14, scRNA-seq n=10). In addition, we re-sampled a subset of these patients for transcriptome analyses at 3 (n=7) and 12 months (n=5) post-infection and followed the expression of differentially regulated genes over time. Genome-wide DNA methylation analysis revealed 3,112 differentially methylated regions between COVID-19 patients and controls. Hypomethylated regions affected immune regulatory genes, while hypermethylated regions were associate

SUBMITTER: Marey Messingschlager 

PROVIDER: S-SCDT-10_1038-S44321-025-00215-5 | biostudies-other |

REPOSITORIES: biostudies-other