Molecular determinants of cardiac lymphatic dysfunction in a chronic pressure-overload model

ABSTRACT: Cardiac lymphatic alterations and insufficient lymphatic drainage have been found in cardiovascular diseases (CVDs). To unravel the mechanisms underlying lymphatic dysfunction, we applied single-cell (sc) analyses in murine heart failure (HF) models. Transaortic constriction (TAC) in C57BL/6J and BALB/c mice modelled chronic pressure-overload-induced cardiac hypertrophy and HF, respectively. Cardiac lymphatic (LEC) and blood vascular endothelial cells (BEC) were analysed by scRNAseq, and targets validated by immunohistochemistry and human LEC cultures. While LEC profiles were comparable between strains in healthy mice, we found expansion of lymphatic capillaries and loss of valves post-TAC only in BALB/c. Differentially-expressed gene (DEG) analysis revealed a reduction post-TAC only in

SUBMITTER: Coraline Heron 

PROVIDER: S-SCDT-10_1038-S44321-025-00345-W | biostudies-other |

REPOSITORIES: biostudies-other