Systematic evaluation of blood contamination in nanoparticle-based plasma proteomics

ABSTRACT: Circulating blood proteomics enables minimally invasive biomarker discovery. Nanoparticle-based circulating plasma proteomics studies have reported varying number of proteins (ca 2000-7000), but it's unclear whether higher protein number is more informative. Here, we first develop OmniProt - a silica-nanoparticle workflow optimized through systematic evaluation of nanoparticle types and protein corona formation parameters. Next, we present an Astral spectral library for 10,109 protein groups. Using Astral with 60 sample-per-day throughput, OmniProt identifies ca 3000 to 6000 protein groups from human plasma. Platelet/erythrocyte/coagulation-related contamination artificially elevates protein identifications and compromises quantification accuracy in nanoparticle-enriched samples. Through c

SUBMITTER: Tiannan Guo 

PROVIDER: S-SCDT-10_1038-S44321-025-00346-9 | biostudies-other |

REPOSITORIES: biostudies-other