Maternal mRNA deadenylation and allocation via Rbm14 condensates facilitates vertebrate blastula development

ABSTRACT: Early embryonic development depends on proper utilization and clearance of maternal transcriptomes. How these processes are spatiotemporally regulated remains unclear. Here we show that nuclear RNA-binding protein Rbm14 and maternal mRNAs co-phase separate into cytoplasmic condensates to facilitate vertebrate blastula-to-gastrula development. In zebrafish, Rbm14 condensates were highly abundant in blastomeres and markedly reduced after prominent activation of zygotic transcription. They concentrated at spindle poles by associating with centrosomal -tubulin puncta and displayed mainly asymmetric divisions with a global symmetry across embryonic midline in 8-cell and 16-cell embryos. Their formation was dose-dependently stimulated by m6A, but repressed by m5C, epigenetic modifications. Furt

SUBMITTER: Dr. Yue Xiao 

PROVIDER: S-SCDT-10_15252-EMBJ_2022111364 | biostudies-other |

REPOSITORIES: biostudies-other