Arrayed Genome-Wide Perturbation Screen Identifies Hnrnpk As Rate-Limiting for Prion Propagation

ABSTRACT: A defining characteristic of mammalian prions is their capacity for self-sustained propagation. Theoretical considerations and experimental evidence suggest that prion propagation is modulated by cell-autonomous and non-autonomous modifiers. Using a novel quantitative phospholipase protection assay (QUIPPER) for high-throughput prion measurements, we performed an arrayed genome-wide RNA interference (RNAi) screen aimed at detecting cellular host-factors that can modify prion propagation. We exposed prion-infected cells in high-density microplates to 35'364 ternary pools of 52'746 siRNAs targeting 17'582 genes representing the majority of the mouse protein-coding transcriptome. We identified 1191 modulators of prion propagation. While 1151 modified the expression of both the PrPSc pathologi

SUBMITTER: Ms. Merve Avar 

PROVIDER: S-SCDT-10_15252-EMBJ_2022112338 | biostudies-other |

REPOSITORIES: biostudies-other