Biochemically validated structural model of the 15-subunit intraflagellar transport complex IFT-B

ABSTRACT: Cilia are ubiquitous eukaryotic organelles impotant for cellular motility, signalling and sensory reception. Cilium formation requires intraflagellar transport of structural and signalling components and involves 22 different proteins organized into intraflagellar transport (IFT) complexes IFT-A and IFT-B that are transported by molecular motors. The IFT-B complex constitutes the backbone of polymeric IFT trains carrying cargo between the cilium and the cell body. Currently, high-resolution structures are only available for smaller IFT-B sub-complexes leaving >50% structurally uncharacterized. Here, we used Alphafold to structurally model the 15-subunit IFT-B complex. The model was validated using crosslinking/mass-spectrometry data on reconstituted IFT-B complexes, X-ray scattering in

SUBMITTER: Narcis, A Petriman 

PROVIDER: S-SCDT-10_15252-EMBJ_2022112440 | biostudies-other |

REPOSITORIES: biostudies-other