Promoter-pervasive transcription causes RNA polymerase II pausing to boost DOG1 expression in response to salt

ABSTRACT: Eukaryotic genomes are pervasively transcribed by RNA polymerase II. Yet, the molecular and biological implications of such a phenomenon are still largely puzzling. Here, we describe non-coding RNA transcription upstream of the Arabidopsis thaliana DOG1 gene, which governs salt stress responses and is a key regulator of seed dormancy. We find that expression of the DOG1 gene is induced by salt stress, thereby causing a delay in seed germination. We uncover extensive transcriptional activity on the promoter of the DOG1 gene, which produces a variety of lncRNAs. These lncRNAs, named PUPPIES, are co-directionally transcribed and extend into the DOG1 coding region. We show that PUPPIES RNAs respond to salt stress and boost DOG1 expression, resulting in delayed germination. This positive role o

SUBMITTER: Mr. Miguel Montez 

PROVIDER: S-SCDT-10_15252-EMBJ_2022112443 | biostudies-other |

REPOSITORIES: biostudies-other