Condensate functionalization with microtubule motors directs their nucleation in space and allows manipulating RNA localization

ABSTRACT: The localization of RNAs in cells is critical for many cellular processes. Whereas motor-driven transport of ribonucleoprotein (RNP) condensates plays a prominent role in RNA localization in cells, their study remains limited by the scarcity of available tools allowing to manipulate condensates in a spatial manner. To fill this gap, we reconstitute in cellula a minimal RNP transport system based on bioengineered condensates, which were functionalized with kinesins and dynein-like motors, allowing for their positioning at either the cell periphery or centrosomes. This targeting mostly occurs through the active transport of the condensate scaffolds, which leads to localized nucleation of phase-separated condensates. Then, programming the condensates to recruit specific mRNAs is able to shift

SUBMITTER: Dr. Audrey COCHARD 

PROVIDER: S-SCDT-10_15252-EMBJ_2023114106 | biostudies-other |

REPOSITORIES: biostudies-other