APC/C-dependent degradation of Spd2 regulates centrosome asymmetry in Drosophila neural stem cells

ABSTRACT: A functional centrosome is vital for the development and physiology of animals. Among numerous regulatory mechanisms of the centrosome, ubiquitin-mediated proteolysis is known to be critical for the precise regulation of centriole duplication. However, its significance beyond centrosome copy number control remains unclear. Using an in vitro screen for centrosomal substrates of the APC/C ubiquitin ligase in Drosophila, we identify several conserved pericentriolar material (PCM) components, including the inner PCM protein Spd2. We show that Spd2 levels are controlled by the interphase-specific form of APC/C, APC/CFzr, in cultured cells and developing brains. Increased Spd2 levels compromise neural stem cell-specific asymmetric PCM recruitment and microtubule nucleation at interphase centroso

SUBMITTER: Dr. Yuu Kimata 

PROVIDER: S-SCDT-10_15252-EMBR_202255607 | biostudies-other |

REPOSITORIES: biostudies-other