Calculation of ATP production rates using the Seahorse XF Analyzer

ABSTRACT: Oxidative phosphorylation and glycolysis are the dominant ATP-generating pathways in mammalian metabolism. The balance between these two pathways is often shifted to execute cell-specific functions in response to stimuli that promote activation, proliferation, or differentiation. However, measurement of these metabolic switches has remained mostly qualitative, making it difficult to discriminate between healthy, physiological changes in energy transduction or compensatory responses due to metabolic dysfunction. We therefore present a broadly applicable method to calculate ATP production rates from oxidative phosphorylation and glycolysis using Seahorse XF Analyzer data. We quantify the bioenergetic changes observed during macrophage polarization as well as cancer cell adaptation to in vitr

SUBMITTER: Mr. Brandon, R. Desousa 

PROVIDER: S-SCDT-10_15252-EMBR_202256380 | biostudies-other |

REPOSITORIES: biostudies-other